In vitro fertilization of mons ova obtained by three ways and their cleavage in commercially and chemically defined media

Abstract EN

In this study ova were collected from the regularly mated, pseudopregnant, and superovulated females Balblc or C57/J6 mice.

The ova were in vitro fertilized by sperms collected from the vas deferens and epididymis of male mice in fertilization medium (FM).

The fertilized ova were cultured in four types of media (MI6, M2, MCM, KSOM).

The results have shown that in in vitro fertilization and early embryonic development depend on the way the ova were collected and the culture media used.

The ova collected from regular mating were superior to those collected from pseudopregnant females or superovulated ones as regards to their ability for in vitro development (35.9%, 8%, 12.8%) respectively.

The different culture media used in this study had different abilities for the maintenance of development of ova (MCM=63.8%, M2=53.1%, MI6=9.4, and KSOM=16.5%) obtained by the three methods of collection.

Superovulated females produce larger number of normal and abnormal ova which had lower capabilities for in vi tro embryonic development compared to those obtained from pseudopregnant or normally mated females.