Separation of Creatine Kinase isoenzymes in the serum and white blood cells and detection it's activities in the white Rabbits with induced Diabetes

Abstract EN

Diabetes Mellitus is a common disease caused by either a deficiency in the secretion of insulin from β-cells or abnormal insulin receptor will lead to physiological and biochemical changes in different part of the body including the blood, there fore this study was included 22 rabbits induced diabetes mellitus (DM) in which injuction the alloxan under skin.

Isoenzyme of creatine kinase (MM-CK, BBCK and MB-CK) were separated by column of DEAE-cellules gel on serum of 22 normal rabbits and the same number with induced DM. The aim of this project is seperation Isoenzyme of creatine kinase (MM-CK, BB-CK and MB-CK) from serum and (WBCs) and determination the effected level influenced by oxidative stress rabbits induced (DM), and we showed that the activity of MM-CK and MB-CK were influenced with DM.

The BB-CK was resistance the changes in concentrations with the control group. Before starting the evaluation, it was prepared the (WBCs) from the rabbits (22 control and 22 patients). In isoenzyme of creatine kinase (MM-CK, BB-CK and MB-CK) results showed that the diabetes mellitus as more effective on MM-CK and MBCK (74.6 ± 14) and, (6.3 ± 1.2), respectively, than on the BB-CK (2.6 ± 0.5). The results of the study showed that there are significantly decreased in level of activity of creatine kinase in patients (for serum 80.6 ± 22) IU / L compared with controls (105.5 ± 38 ) IU / L in both serum and, (WBCs). Finally, this study was illustrating that there were no significant differences (P ≤ 0.01) between the levels of serum and (WBCs) in each groups for the parameters studied (isoenzyme creatine kinase).