Plasmid curing by using essential oil of rosmarinus officinalis

Abstract EN

Background : Rosmarinus officinalis essential oil is a natural product it has been reported to be much safer than antibiotic, and has abroad spectrum activity against gram positive and gram negative stain bacteria.

On the other hand it has been reported that the oil has no toxicity and lethality on laboratory white mice therapeutic dose range.

Objective : Used of essential oil of Rosemary in plasmid curing for R-plasmid (antibiotic resistance plasmid).

Methods : Used of minipreparation of plasmid DNA and cured plasmid with essential oil by concentration (100µg / ml), the detection was performed by agarose gel electrophoresis.

Results : The essential oil has an active ability to curing plasmid as it is detected by DNA electrophoresis and lost the phenotype of Antibiotic resistance.

Conclusion : In this study, essential oil of Rosemary used for plasmid curing in active way against Escherichia coli pBR322 which contain R-plasmid which is resistant to Ampincilin, Tetracycline and Streptomycin antibiotics this help to used essential oil as cofactor for antibiotics.

Key words : Rosmarinus officinalis, essential oil, plasmid curing.