Apoptosis and apoptotic factors (bcl2 and bax protein)‎ in recurrent benign nasal polyp

Abstract EN

Apoptosis is an evolutionarily conserved form of cell death.

In this respect, molecular characterization of the mechanisms involved in the regulation and execution of apoptosis could provide crucial information for understanding the pathogenesis of nasal polyps.

The aims were as follow : 1.

To study the percentage of apoptotic cells of nasal polyp by TUNEL assays (the recognized DNA fragmentation within the cells).

2.

To study the role of versus reactions between anti apoptotic Bcl2 and pro apoptotic Bax proteins in surviving cells of nasal polyp.

Polypectomy for 42 patient with recurrent nasal polypoidal masses was taken with a small piece of normal inferior turbinate as a control tissue & kept separately as biopsy for TUNEL assay for detection of percentage of apoptosis & for the detection of the percentage of Bcl2 & Bax protein immunohistochemical study.

The Results showed that.

The percentage of apoptotic cells in non-nasal polyp tissue were significantly higher than the percentage of apoptotic cells in nasal polyp tissue (73.94±15.12, 18.86±6.56), (with P-value<0.001).

The percentage of Bcl2 protein in non-nasal polyp tissue was significantly lower than the percentage of Bcl2 protein in nasal polyp tissue (20.25±4.46.12, 71.27±17.26) respectively (with P-value <0.001) while the percentage of Bax protein in non-nasal polyp tissue was significantly higher than the percentage of Bax protein in nasal polyp tissue (85.78.±5.42, 33.14±10.13) respectively, with P-value <0.001.

The apoptotic mechanism is lower in the recurrent nasal polyp tissue than in the normal (inferior turbinate) tissue.

This may have a rule in the aetiopathology of the recurrence of nasal polyps.