A clinico-bacteriological study of the interrelation of corynebacterium urealyticum and struvite urolithiasis

Abstract EN

Three hundred twenty urine samples were collected from patients suffering urinary tract affection.

Only alkaline sample were selected which formed 86 specimens yielded 8 isolates of Cory nebacterium urealyticum.

All the 8 isolates were identical regarding cultural characteristics and biochemical reactions.

Results of the studied virulence factors expression by Cory nebacterium urealyticum showed that urease was the most important factor in pathogenicity.

An experimental study was created to assess the ability Struvite stone formation by Cory nebacterium urealyticum during its growth in human urine; it was found that there was gradual increase in urine pH with associated elevation of ammonium ion concentration accompanied by bacterial growth and striated crystals which appeared grossly as a white precipitate at the bottom of the testing tube after an overnight incubation in human urine.

This observation was compared to the failure of E.

coli and control urine to increase the urine pH or ammonium concentration in sterile urine specimens, together with the inability form stupide crystals when incubated under similar experimental Conditions.

The effects of the proton – pump inhibitors : omeprazole and lansoprazole on growth and pathogenicity of Cory nebacterium urealyticum were examined, it was obvious that these compound markedly affected urease activity in a dose-dependent manner and the bacterial growth was significantly (p<0.001) inhibited by an increasing concentrations of omeprazole.