Isolation and characterization of probionibacteriumacnes from acne vulgaris patients

Abstract EN

In this study, 110 skin swabs were obtained from patients suffering from acne vulgaris from consultation unit in Merjan teaching Hospital, and allergy specialized center in Al-Hilla from both It sexes.

Was found that acne vulgaris was higher in female (62.7%) and more prevalence in (16-20) years patients and lower in older especially after 30 years age where there is only one case.

Three isolates of anaerobic gram positive Probionibacterium acnes were identified and diagnosis by culture and biochemical tests, all bacterial strains possessed capsule and produce lipase and protease enzymes and contained the third colonization factor antigen (CFA-III) while only one strain produce a narrow zone of β-hemolysis.

The effect of some antibiotics on Probionibacterium acnes was investigated, and the results showed that all isolates were sensitive to Tetracycline, Deoxcycline, Chloromphenecol, Neomycine, and Kephalexin, while they were resistant to Erythromycin, Fusidic acid, Vencomycin, Lincomycin and Gentamycin.

Also retinoic acid effect on bacterial growth was studied.

It was stated that lactic at high concentration 20 μg / ml could cause inhibition of Probionibacterium acnes growth.

Also temperature over 50 C °would kill the bacteria