Detecting leukocytospermia by melyoperoxidase staining method for patients of baghdad ivf institute

Abstract EN

The objective of this study is to detect the leukocytes concentration in the semen of infertile and fertile patients The study include three hundred and forty men referred to IVF Institute, Baghdad Teaching Hospital,.

They are 290 infertile men and 50 fertile men (control).

Semen analysis was done and the patients divided into subgroups according to male infertility factors .Seminal myeloperoxidase cytochemical test (Endtz method) performed for all specimens to differentiate between the leukocytes and other round cells.

The concentration of Leukocytes were account using high power field (HPF) method and Endtz.

The results revealed Leukocyte concentration in normospermic patients (control) by using HPF method was significantly (P<0.01) higher than Endtz method.

In oligospermic, asthenospermic and oligo-astheno-terato sperm men, the mean of leukocyte concentration by using HPF was significantly lower (P<0.05) compared to using Endtz method.

There was a highly significant (P<0.01) decrease in the number of leukocytes by using Endtz method compared with the round cells detected by using HPF method.

It was concluded that myeloperoxidase (Endtz) method can be detect the leukocytes concentration in the semen and differentiate them from other round cells.

This method is easy to performed, complete with short time with high accuracy, not cost and can sustain the correct treatment.