Effect of purified pyocyanine produced by pseudomonas aeruginosa on other microbes

Abstract EN

In this study Pseudomonas aeruginosa was isolated and purified from burns and wounded patients who attending and residents in some Baghdad hospitals.

The high pyocyanine production isolates were selected and the identification of Pseudomonas aeruginosa according to biochemical & morphological tests were done.

The effect of physical conditions (pH and temperature) on pyocyanine production was studied.

The results showed that the optimal pH and temperature were (7.5 ; 37°C) Respectively.

The different concentrations of (0, 25, 50, 100, 200, 300) μg / ml Showed the Staphylococcus and streptococcus were inhibited at 50 μg / ml, while the clostridium at 300 μg / ml, but the Genus Pseudomonas had not been affected at all the concentrations of the pigment.