Bacterial and mycotic causes of subclinical bovine mastitis

Abstract EN

A total of 500 quarter milk samples were collected from 125 apparently healthy animals (85 buffaloes and 40 dairy cattle) of different breeds from different farms at Ismailia Governorate, all quarter milk samples were subjected to California mastitis test(CMT), samples with CMT scores ± ,+ ,++ and +++ were subjected to both bacteriological and mycological examinations.

The use of CMT proved that 52.3% of examined samples were positive to subclinical mastitis.

Microbiological examination showed that the most prev-alent pathogenic organisms isolated were S.

aureus (32.3%), S.

uberis (13%), S.

epidermidis (12%), S.

dysgalactiae (10,2%), E.

coli (10.2%), S.

bovis (6.2%), Klebsiella pneumoniae (3.4%), E.

fecalis (3.1%), E.

facium (1.5%) and Proteus vulgaris (1.4%).

Mycotic infection were recorded in 23 quarter milk samples (6.5%) including Candida albicans (3.4%), Cladosporium spp.(1.9%), Asper-gillus niger(0.56%) and Rhodotorula spp.

(0.56%).

PCR protocol used for amplification and detection of both 16S rRNA genes and (coa) gene of Staph.

aureus isolates to confirm diagnosis of S.

aureus as most common isolates and their pathogenicity