Rapid quantification of mirtazapine and desmethyl mirtazapine in human plasma by LC-ESI-MSMS: Application to a bioequivalence study

Abstract EN

A high-throughout bioanalytical method based on a solid-phase extraction (SPE), and rapid liquid chromatography-tandem mass spectrometry (LC-MS / MS) analysis has been developed and validated for the quantification of mirtazapine (MRT) and desmethyl mirtazapine (DMRT) in heparin zed human plasma.

Plasma samples, without a drying and reconstitution step, were extracted by a simple SPE.

The analytes and imipramine (internal standard, IS) chromate-graphed on a Betasil-C18 column.

The total chromatographic run time was 1.8 min per sample.

Response was a linear function of concentration in the range 0.1-100.0 ng /ml, with correlation coef-