Determination of phenylephrine hydrochloride and chlorpheniramine maleate in binary mixture using chemometric-assisted spectrophotometric and high-performance liquid chromatographic-UV methods
Author
Source
Journal of Saudi Chemical Society
Issue
Vol. 14, Issue 1 (31 Mar. 2010), pp.15-21, 7 p.
Publisher
Publication Date
2010-03-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Four methods have been developed for the simultaneous determination of phenylephrine hydrochloride and chlorpheniramine maleate without previous separation.
In the first method both drugs are determined using first derivative UV spectrophotometry, with zero-crossing measurement.
The second method depends on first derivative of the ratios spectra.
The third method describes the use of multivariate spectrophotometric calibration for the simultaneous determination of the analyzed binary mixture where the resolution is accomplished by using partial least squares (PLS) regression analysis.
In the fourth method (HPLC), a reversed-phase column and a mobile phase of methanol : water : acetonitrile (80 : 12 : 8 v /v /v /) at 0.9 ml /min flow rate have been used to separate both drugs with a UV detection at 270 nm.
All the proposed methods are extensively validated.
They have the advantage to be economic and time saving.
All the described methods can be readily utilized for analysis of pharmaceutical formulations.
The results obtained using the proposed methods are statistically analyzed and compared with some reported methods.
American Psychological Association (APA)
al-Shalan, Nurah H.. 2010. Determination of phenylephrine hydrochloride and chlorpheniramine maleate in binary mixture using chemometric-assisted spectrophotometric and high-performance liquid chromatographic-UV methods. Journal of Saudi Chemical Society،Vol. 14, no. 1, pp.15-21.
https://search.emarefa.net/detail/BIM-250656
Modern Language Association (MLA)
al-Shalan, Nurah H.. Determination of phenylephrine hydrochloride and chlorpheniramine maleate in binary mixture using chemometric-assisted spectrophotometric and high-performance liquid chromatographic-UV methods. Journal of Saudi Chemical Society (Mar. 2010), pp.15-21.
https://search.emarefa.net/detail/BIM-250656
American Medical Association (AMA)
al-Shalan, Nurah H.. Determination of phenylephrine hydrochloride and chlorpheniramine maleate in binary mixture using chemometric-assisted spectrophotometric and high-performance liquid chromatographic-UV methods. Journal of Saudi Chemical Society. 2010. Vol. 14, no. 1, pp.15-21.
https://search.emarefa.net/detail/BIM-250656
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 20-21
Record ID
BIM-250656