A study of adsorptive stripping voltammetric behavior of ofloxacine antibiotic in the presence of Fe(III)‎ and its determination in tablets and biological fluids

Abstract EN

Square-wave voltammetry was used to explore the adsorption property of ofloxacine complex with iron ion on the hanging mercury drop electrode (HMDE).

By employing the adsorptive stripping voltammetry approach, a sensitive electro analytical method for the quantitative analysis of ofloxacine antibiotic was achieved.

A well-developed voltammetry peak was obtained in pH 7.5 Britton-Robinson buffer (B-R buffer) at -1400 mV.

The cyclic voltammetry studies indicated that the reduction process was irreversible and primarily controlled by adsorption.

An investigations of the variation of adsorptive voltammetry peak current with supporting electrolyte, pH, accumulation time, accumulation potential, ion concentration, scan rate, pulse amplitude, SW frequency, working electrode area and convection rate has resulted in the recognition of optimal experimental conditions for ofloxacine analysis.

The studied electro analytical signal showed a linear response for ofloxacine in the concentration range 5 x 10-7 to 1.7 x 10-5 mol P1 (r = 0.999).

A limit of detection of 1.1 x 10"8moir1 (3.98 ppb) with relative standard deviation of 1.21 RSD% and mean recovery of 99.6% were obtained.

Possible interferences by several substances usually present in pharmaceutical formulation were also evaluated.

The analytical quantification of ofloxacine in commercially available pharmaceutical formulation was performed and compared with data from HPLC technique.