Detection and prevalence of polyoma virus bk among Iranian kidney transplant patients by a novel nested-PCR
Joint Authors
Ravanshad, M.
Hosseini, S. Y.
Ghanbari, R.
Shenagari, M.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 12, Issue 6 (30 Nov. 2010), pp.631-635, 5 p.
Publisher
Publication Date
2010-11-30
Country of Publication
United Arab Emirates
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background : BK virus is an increasingly recognized pathogen in transplant recipient patients associated with nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimens in renal transplant recipients.
This study develops a sensitive PCR method to detect the vermeil and viruria as well as the incidence of BK virus infection in renal transplant recipients.
Methods : A nested PCR method was developed and a total of 45 paired serum and urine samples from renal transplant recipient patients were collected and tested with the developed assay.
Results : The threshold of the developed detection assay was 10 copies / μl of BKV DNA in samples.
Our results also indicated that about 40% of the urine and 26.7% of serum samples were positive for BKV in renal transplant patients in this study.
Conclusion : this Nested-PCR method was found a specific, sensitive and simple procedure for detection of viruria and vermeil of BK virus in renal transplant recipients.
American Psychological Association (APA)
Shenagari, M.& Ravanshad, M.& Hosseini, S. Y.& Ghanbari, R.. 2010. Detection and prevalence of polyoma virus bk among Iranian kidney transplant patients by a novel nested-PCR. Iranian Red Crescent Medical Journal،Vol. 12, no. 6, pp.631-635.
https://search.emarefa.net/detail/BIM-252101
Modern Language Association (MLA)
Ravanshad, M.…[et al.]. Detection and prevalence of polyoma virus bk among Iranian kidney transplant patients by a novel nested-PCR. Iranian Red Crescent Medical Journal Vol. 12, no. 6 (Nov. 2010), pp.631-635.
https://search.emarefa.net/detail/BIM-252101
American Medical Association (AMA)
Shenagari, M.& Ravanshad, M.& Hosseini, S. Y.& Ghanbari, R.. Detection and prevalence of polyoma virus bk among Iranian kidney transplant patients by a novel nested-PCR. Iranian Red Crescent Medical Journal. 2010. Vol. 12, no. 6, pp.631-635.
https://search.emarefa.net/detail/BIM-252101
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 634-635
Record ID
BIM-252101