Detection and prevalence of polyoma virus bk among Iranian kidney transplant patients by a novel nested-PCR

Abstract EN

Background : BK virus is an increasingly recognized pathogen in transplant recipient patients associated with nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimens in renal transplant recipients.

This study develops a sensitive PCR method to detect the vermeil and viruria as well as the incidence of BK virus infection in renal transplant recipients.

Methods : A nested PCR method was developed and a total of 45 paired serum and urine samples from renal transplant recipient patients were collected and tested with the developed assay.

Results : The threshold of the developed detection assay was 10 copies / μl of BKV DNA in samples.

Our results also indicated that about 40% of the urine and 26.7% of serum samples were positive for BKV in renal transplant patients in this study.

Conclusion : this Nested-PCR method was found a specific, sensitive and simple procedure for detection of viruria and vermeil of BK virus in renal transplant recipients.