Species identification and strain typing of candida isolates by pcr-rflp and rapd-pcr analysis nosocomial infections
Joint Authors
Fahami, Sh.
Kordbacheh, P.
Moazeni, M.
Mahmoodi, M.
Mirhendi, H.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 12, Issue 5 (30 Sep. 2010), pp.539-547, 9 p.
Publisher
Publication Date
2010-09-30
Country of Publication
United Arab Emirates
No. of Pages
9
Main Subjects
Abstract EN
Background : since the incidence of nosocomial Candida infections have been increasing in parallel with the raising in the number of patients involving in predisposing factors, determining the sources and the ways of acquisition of infection is necessary as an efficient strategy for controlling the diseases.
The aim of this study is identification and strain typing of Candida strains isolated from hospitals to facilitate tracing the sources of infections in hospitalized patients in addition to assess the discriminatory power of some random primers by using RAPD analysis.
Methods : samples were collected from patients who were hospitalized in oncology, intensive care unit (ICU), and organ transplants wards of the Shohadaye-Tajrish Hospital affiliated to Tehran University of Medical Sciences, Tehran, Iran, and their environments.
The yeasts were isolated on CHRO Magyar Candida.
Species identification was performed by PCR-amplification of ITS1-5.8SrDNA–ITS2 region followed by restriction digestion with the enzyme MspI.
To determine the probable origin of candida infections, in case of each patient whose the clinical and relevant environmental isolates were identified as the same species, a set of eight primers namely number 1 to 8, were applied in RAPD-PCR to find out the possible homogeny or variation within the isolated strains.
Results : one hundred and four Candida strains were identified.
The most common species was C.
albinos (57.5%) followed by C.
tropical is (13.5%), C.
globate (12.%), C.
parapsilosis (8.65%), C.
famata (3.8%), C.
Kruse (1.9%), C.
guilliermondii (0.96%).
and C.
lusitaniae (0.96%).
While the source of infection for three patients were not determined by RAPD analysis, interpretable results from RAPD-typing of Candida species isolated from 8 / 18 of cases implied that the infections might originate from the exogenous sources.
Moreover, according to the function of each primer, primer No.
1 was determined as a best primer for typing of Candida albinos strains.
Conclusion : the species of yeast isolates were determined by PCR-RFLP.
It was found that RAPD assay can point out the genomic variability within the Candida species.
Besides, the method could show a probable relationship between acquired infections and their sources.
American Psychological Association (APA)
Fahami, Sh.& Kordbacheh, P.& Moazeni, M.& Mahmoodi, M.& Mirhendi, H.. 2010. Species identification and strain typing of candida isolates by pcr-rflp and rapd-pcr analysis nosocomial infections. Iranian Red Crescent Medical Journal،Vol. 12, no. 5, pp.539-547.
https://search.emarefa.net/detail/BIM-252224
Modern Language Association (MLA)
Fahami, Sh.…[et al.]. Species identification and strain typing of candida isolates by pcr-rflp and rapd-pcr analysis nosocomial infections. Iranian Red Crescent Medical Journal Vol. 12, no. 5 (Sep. 2010), pp.539-547.
https://search.emarefa.net/detail/BIM-252224
American Medical Association (AMA)
Fahami, Sh.& Kordbacheh, P.& Moazeni, M.& Mahmoodi, M.& Mirhendi, H.. Species identification and strain typing of candida isolates by pcr-rflp and rapd-pcr analysis nosocomial infections. Iranian Red Crescent Medical Journal. 2010. Vol. 12, no. 5, pp.539-547.
https://search.emarefa.net/detail/BIM-252224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 546-547
Record ID
BIM-252224