Polymerase chain reaction for differentiation of pasteurella multocida isolates from turkeys in comparison to strains incorporating in fowl cholera vaccine

Abstract EN

In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study.

Strains included 4 vicinal reference strains of Pasteur Ella multocida, CU strain and 4 field isolates of Pasteur Ella multocida isolated from diseased turkeys which were identified biochemically and serologically as A :1, A : 3, A3 x 4 and D : 11.

The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates.

The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain.

This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer.

Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A : 1, A : 3, D : 12 and untamable isolates.

Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A : 1, A : 3 and D : 12 field strains but it could not be protect mice against untamable isolates.