Effectiveness of microwave sterilization on soft lining material
Other Title(s)
فعالية التعقيم الميكروويف على المواد بطانة لينة
Joint Authors
Mustafa, Iman A.
Jawad, Inas A.
Abd al-Qadir, Asma A.
Source
Issue
Vol. 10, Issue 1 (30 Jun. 2010), pp.133-142, 10 p.
Publisher
University of Mosul College of Dentistry
Publication Date
2010-06-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract AR
الأهداف : تقييم فاعلية التعقيم بالتشعيع بالموجات المايكروية لمادة التبطين الطرية لطقم الأسنان.
طريقة العمل : تم تحضير ستون عينة من مادة التبطين الطرية لطقم الأسنان تبعا للطريقة القياسية و عقمت بجهاز التعقيم بالموصدة.
قسمت العينات إلى أربعة مجاميع و تم تحضين عينات كل مجموعة مع أحد أنواع الأحياء المجهرية المختبرة التالية : المكورات العنقودية، عصيات سبتلس، زوائف ايروجينوزا، و المبيضات البيضاء.
عوملت عينات كل مجموعة بالتشعيع بالموجات الميكروية 540 وات لستة دقائق و قورنت مع مجموعة السيطرة.
النتائج : أظهرت الدراسة أن هناك تأثير مطهر واضح على جميع الأحياء المجهرية المختبرة بعد 48 ساعة تحضين، و تأثير معقم على المبيضات البيضاء بعد 48 ساعة تحضين و 7 أيام تحضين.
Abstract EN
The aim of this study was to evaluate the effectiveness of microwave irradiation sterilization on Molloplast –B soft denture liner.
Materials and Methods : Sixty specimens of Molloplast –B soft den-ture liner were fabricated in a standardize procedure and autoclaved.
The total 60 specimens were di-vided into 4 groups.
Each group has 15 specimens inculated with Brain Heart Infusion Broth (BHI) media containing one of the tested microorganisms (Staphylococcus aureus, Bacillus subtilis, Pseudo-monas aeroginosa and Candida albicans).
Then the 15 specimens in each group was further divided into 3 subgroups: group C (positive control); 5 none irradiated specimens, group D (dry microwave treat-ment); 5 specimens placed in a dry beaker and microwave irradiated at 540W for 6 minutes, group W (wet microwave treatment; 5 specimens immersed in distalled water and irradiated in the same manner as group D.
After incubation of all specimens for 24 hours at 37°C, the specimens were got vortex and then the replicated specimens (100μL) of suspensions were plated on 4 selective media appropriate for each organism.
All plates were incubated at 37°C for 48 hours.
After incubation, colonies were counted.
Further 7 days incubation for microwaved specimens was done to verify the effectiveness of dry and wet microwave sterilization.
Results : Significant reduction in cfu/ml of all microorganisms was observed at 48 hours.
No growth of C.
albicans was recorded at 48 hours and after 7 days incuba-tion.
Conclusions : Microwave irradiation at 540W for 6 min in dry and wet conditions was proved to be effective in the disinfection of soft lining material specimens contaminated with Staph.
aureus, Ps.
aeruginosa and B.
subtilis.
Wet treatment was more effective than dry one.
Dry and wet microwave treatment sterilized specimens contaminated with C.
albicans.
American Psychological Association (APA)
Mustafa, Iman A.& Jawad, Inas A.& Abd al-Qadir, Asma A.. 2010. Effectiveness of microwave sterilization on soft lining material. al-Rafidain Dental Journal،Vol. 10, no. 1, pp.133-142.
https://search.emarefa.net/detail/BIM-253530
Modern Language Association (MLA)
Mustafa, Iman A.…[et al.]. Effectiveness of microwave sterilization on soft lining material. al-Rafidain Dental Journal Vol. 10, no. 1 (Nov. 2010), pp.133-142.
https://search.emarefa.net/detail/BIM-253530
American Medical Association (AMA)
Mustafa, Iman A.& Jawad, Inas A.& Abd al-Qadir, Asma A.. Effectiveness of microwave sterilization on soft lining material. al-Rafidain Dental Journal. 2010. Vol. 10, no. 1, pp.133-142.
https://search.emarefa.net/detail/BIM-253530
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 140-142
Record ID
BIM-253530