Disinfection of extracted teeth for dental researches

Abstract EN

To determine the effectiveness of different disinfection methods on extracted human teeth using five types of bacteria Proteus species, Escherichia coli, Kelebsiella species, Staphylococcus aureus, Streptococcus mutans.

Materials and Methods : In this study extracted non-carious teeth were divided into five groups according to the type of bacteria that were inoculated inside the pulp chambers.

Each group of the teeth were subdivided into six groups; group A : teeth were immersed in 2.5% NaOCl for 1week, group B : teeth were immersed in 1% NaOCl for 1week.

Group C : teeth were autoclaved at 121C for at 15 Ibs psi for 15 minutes, group D : teeth disinfected using microwave for 3 minutes, group E : teeth disinfected using microwave for 6 minutes, group F: control group in which the teeth im-mersed in normal saline for seven days at room temperature.

Each tooth aseptically placed in individual test tube with growth media.

Simples were examined after 24h.

Results : showed that autoclave, mi-crowave (when used at sex and three minutes), sodium hypochlorite at 2.5% prevented the growth completely in all types of the bacteria that were used to infect the teeth involved in this study.