The detrimental influence of centrifugation force on seminal fluid analysis parameters and pregnancy rates of human spermatozoa in infertile patients after intra-uterine insemination technique

Abstract EN

This study was designed to evaluate the effect of centrifugation force on seminal fluid analysis parameters and pregnancy rate in infertile subjects undergoing intrauterine insemination (IUI) technique.

One hundred infertile patients enrolled in this study and semen samples were analyzed before and after in vitro sperm processing by standard semen parameters.

However, the spermatozoa prepared by direct swim-up and centrifugation swim-up technique as sperm preparation methods.

Conversely, HO- solution was performed before and after in vitro sperm preparation by mixing 0.1 ml of semen with 1.0 ml of a 150 mosm/L NaCl as hypo-osmotic solution.

Furthermore, sperm concentration, sperm agglutination, sperm motility, progressive sperm motility, normal sperm morphology, and HOS-test were evaluated according to standard WHO criteria.

For IUI technique, sperm prepared and incubated for 30 minute in 5 % CO2 at 37ºC.

The results of the present study indicate a highly significant (P < 0.001) differences for all sperm functions and sperm HOS-test (P < 0.001) were reported post in vitro sperm activation using direct swim-up and centrifugation swim-up method as compared to pre-activation.

Finally, the best results for SFTs and clinical pregnancy rate were reported for semen samples prepare by direct swim-up with IVF culture medium.