Extraction and purification of urease from proteus mirabilis
Joint Authors
al-Sadi, Narjis Mansur Hadi
al-Jumayli, Isam Fadil A.
al-Rawi, Anis M.
Source
Issue
Vol. 2009, Issue 33 (31 Mar. 2009), pp.138-145, 8 p.
Publisher
University of Babylon College of Sciences
Publication Date
2009-03-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Urease was extracted from uropathogenic P.
mirabilis using Lauria agar and then purified by ion- exchange chromatography using DEAE-cellulose and gel filtration chromatography on Sephacryl S-200 column.
The purification fold was 13.86 and the yield was 45.4 %.
The molecular weight of urease was estimated by gel filtration and SDSpolyacrylamide gel electrophoresis.
The native molecular weight of the enzyme using gel filtration was about 174000 Daltons whereas SDS-polyacrylamide gel electrophoresis revealed three bands and the molecular weight for these bands were (53.70, 31.68, 20.89) kDa respectively.
American Psychological Association (APA)
al-Sadi, Narjis Mansur Hadi& al-Jumayli, Isam Fadil A.& al-Rawi, Anis M.. 2009. Extraction and purification of urease from proteus mirabilis. National Journal of Chemistry،Vol. 2009, no. 33, pp.138-145.
https://search.emarefa.net/detail/BIM-254873
Modern Language Association (MLA)
al-Sadi, Narjis Mansur Hadi…[et al.]. Extraction and purification of urease from proteus mirabilis. National Journal of Chemistry No. 33 (2009), pp.138-145.
https://search.emarefa.net/detail/BIM-254873
American Medical Association (AMA)
al-Sadi, Narjis Mansur Hadi& al-Jumayli, Isam Fadil A.& al-Rawi, Anis M.. Extraction and purification of urease from proteus mirabilis. National Journal of Chemistry. 2009. Vol. 2009, no. 33, pp.138-145.
https://search.emarefa.net/detail/BIM-254873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 144-145
Record ID
BIM-254873
