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Evaluation of stability of cefamandol and ceftazidime with clavulanic acid against extended Spectrum β-lactamase
Other Title(s)
تقييم استقرار سيفامندول و سفتازايدين مع حمض الكلافيونك في الصيف الممتد لبيتا لاكتامايز
Joint Authors
Shawkat, Siham S.
Hamid, Hammudi Ahmad
Source
Iraqi Journal of Pharmaceutical Sciences
Issue
Vol. 19, Issue 2 (31 Dec. 2010), pp.42-47, 6 p.
Publisher
University of Baghdad College of Pharmacy
Publication Date
2010-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract AR
يهدف هذا البحث إلى دراسة فعالية السيفامندول (Cfm) و السيفتازديم (Cfz) بإضافة حامض الكلافجولانك تجاه (E-Coli) المقاومة لمجموعة مضادات البيتالاكتام معزولة من مرضى يعانون من التهاب المجاري البلوية المزمن و قد بينت الدراسة أن الفعالية المثلى لكل من السيفامندول و السيفتازديم تكون بوجود حامض الكلافجولانك و أن ثباتية كل من السيفامندول و السيفتازديم بوجود الحامض تشير إلى إمكانية استخدامهما في معالجة التهاب المجاري البولية الناتجة عن (E-Coli) المقاومة لمضادات البيتالاكتام من خلال طرائق التقييم و التشخيص التي استخدمت في البحث.
Abstract EN
The aim of this study is to evaluate in-vitro activity of Cefamandol (Cfm) and Ceftazidime (Cfz), in combination with Clavulanic acid (CA) against ten complicated multiresistant uropathogenic E.
coli.
One hundred clinical strains were isolated from patients with chronic urinary tract infections (UTIs), these isolates were identified by the Api identification systems.
The antimicrobial susceptibility tests were determined by Kirby-Bauer method, all of them were sensitive to Imipenem (Imp).
Ten strains were chosen for the present study, they were resistant to Ampicillin (Amp), Amoxicillin (Amo), Carbenicillin (Cb), Ticarcillin (Tic), Azlocillin (Azl), Amoxicillin \ Potassium Clavulanate {Augmentin(Amc)}, (Amo \ CA), Ticarcillin \ Potassium Clavulanate {Timentin} (Tic \ CA), Cefazolin (Cfo), Cefaloridin (Cfr), Cefamandol, (Cfm), Cefoxitin, Ceftazidime (Cfz), Cefixime (Cxm), Cefoperazone ( Cfp) and Aztreonam (Atm), also resistant to other antibiotics, Tetracycline (Tc), Chloramphenicol (Cm), Gentamycin (G), Amikacin (Amk), Ciprofloxacin (Cip) and Trimethoprim.
50% of the isolates were resistant to Nalidixic acid and Rifampicin.
The minimum inhibitory concentrations of Cefamandol and Ceftazidime were determined, by tube method.
Transfer of plasmids were done by direct conjugation test to sensitive standard E.
coli, cell free β-lactamases were prepared and detected by macro-iodometric method.
The activity of each cell free ß-lactamases extract against Cfm and Cfz were determined by disks diffusion method (microbiological Masuda method).
Excellent activities were obtained against these strains when Cfm and Cfz, combined with CA, therefore complete zones of inhibition were obtained indicated the prevalence of extended Spectrum β-lactamases in E.
coli.
The stability of Cfm and Cfz in the presence of CA were useful in the treatment of chronic urinary tract infections caused by multiresistant β-lactamase (ESBL) producer E.
coli.
American Psychological Association (APA)
Shawkat, Siham S.& Hamid, Hammudi Ahmad. 2010. Evaluation of stability of cefamandol and ceftazidime with clavulanic acid against extended Spectrum β-lactamase. Iraqi Journal of Pharmaceutical Sciences،Vol. 19, no. 2, pp.42-47.
https://search.emarefa.net/detail/BIM-255683
Modern Language Association (MLA)
Shawkat, Siham S.& Hamid, Hammudi Ahmad. Evaluation of stability of cefamandol and ceftazidime with clavulanic acid against extended Spectrum β-lactamase. Iraqi Journal of Pharmaceutical Sciences Vol. 19, no. 2 (Dec. 2010), pp.42-47.
https://search.emarefa.net/detail/BIM-255683
American Medical Association (AMA)
Shawkat, Siham S.& Hamid, Hammudi Ahmad. Evaluation of stability of cefamandol and ceftazidime with clavulanic acid against extended Spectrum β-lactamase. Iraqi Journal of Pharmaceutical Sciences. 2010. Vol. 19, no. 2, pp.42-47.
https://search.emarefa.net/detail/BIM-255683
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 46-47
Record ID
BIM-255683