Detection of chlamydophila psittaci in chickens by complement fixation test and polymerase chain reaction

Abstract EN

This study was carried out on 68 randomly collected chickens located at Ras Sedr Research Station, Desert Research Center, 68 serum samples were examined serologically by complement fixation test (CFT).

Twenty out of 68 (29.91 %) had antibodies against Chlamydophila pittance.

Ten blood samples of the serologically positive cases were subjected to polymerase chain reaction (PCR) and showed positive results for Chlamydophila pittance at 119 bp.

Therefore PCR was found to be reliable, rapid, sensitive and specific technique for the detection Chlamydophila pittance in birds.

Serologically positive birds did not show any clinical symptoms of disease, but they were in contact with sheep and goat that showed previous abortion and were positive for C.

abort us.

It is recommended to avoid breeding of chickens with other animal species in the same yard because chickens become asymptomatic carrier with shedding of Chlamydophila pittance in their faces and respiratory discharges.