The role of corynebacterial phospholipase D vaccine in activation of macrophages

Abstract EN

The role of phospholipase D toxoid (PLD) vaccine in enhancing killing activity of macrophages was demonstrated in this study.

Four groups of Balb/c mice were vaccinated with different forms of current vaccines against Corynebacterium pseudo tuberculosis (C.

pseudo tuberculosis).

The first group was vaccinated with purified recombinant mutated PLD protein adulated vaccine; the second with formalin inactivated whole cells of C.

pseudo tuberculosis adulated vaccine, the third group with combined bacteria-toxoid adulated vaccine and the fourth was given viable C.

pseudo tuberculosis cells.

Mononuclear peritoneal cells from each vaccinated groups were collected and inoculated intraperitoneally into naïve recipient Balb /c mice that were subsequently challenged by equal number of live C.

pseudo tuberculosis cells.

Killing activity of peritoneal macrophages collected from each recipient group of mice was assayed by cultivation of lysed macrophages on plates of count brain heart agar.

It was reported that the highest killing activity of macrophages were those collected from mice vaccinated with recombinant PLD adulated vaccine that reaches 95 % of phagocytized C.

pseudo tuberculosis living bacteria; where those given viable C.

pseudo tuberculosis bacteria (80 %), then combined vaccine (69.5 %) and the least killing activity was performed by macrophages obtained from bacteria vaccinated animals