Comparison of the combination of recomline and elisa with real-time polymerase chain reaction in the final : diagnosis of toxoplasmosis

Abstract EN

Background : the diagnosis of Toxoplasma genii infection in human can be determined by variable immunological and molecular methods.

The methodology for antibodies detection is well established allowing the identification of serological profile correlated with the stage of the infection.

The recombined Toxoplasma is designed as a confirmation test for serologic diagnosis.

The line assay technique facilitates antibodies detection and identification in a single test.

While real-time PCR has recently been introduce for the diagnosis of toxoplasmosis in a short time.

Patients and Methods : Twenty women who had a history of abortion and infected with acute toxoplasmosis were firstly diagnosed by ELISA IgM Toxoplasma antibodies.

Then the diagnosis was confirmed by recombined Toxoplasma IgM .Finally real time-PCR was done to each woman.

Results : the results showed the utility of combination of ELISA and recombined in the diagnosis of toxoplasmosis than using each test alone in the diagnosis, or using RT-PCR.

The sensitivity of the parallel combination of ELISA and recombined assays was 80 %, while the sensitivity of RT-PCR was only 40 %.

Conclusion : detection of lgM-Toxoplasma antibodies by ELISA lead to false positive results, a confirmatory test like recombined assay which is satisfactory test that offer the same advantage of immunoblotting in the diagnosis of acute toxoplasmosis.

A new tests system that include the combination of ELISA and recombined assays, had high sensitivity compared to the sensitivity of RT-PCR, and can be used in the diagnosis of acute toxoplasmosis.