Isolation and identification of bacteria in dummy sucking children with gastroenteritis : ( comparative study)‎

Abstract EN

Background: Dummy sucking had been associated with many adverse effects on general health of the children, one of them is infection due to contaminations, this study included the isolation and identification of microorganism associated with gastroenteritis dummy sucking infected children to determine the most common microorganism isolated in the sample of study group with age ranged between (1 -36) months from both gender in AL-karama Teaching Hospital between Sep.

2008 to Jan.

2009.

Material and methods: the total studied specimens were sixty five (65) dummy swabs.

Forty five dummy swabs from gastroenteritis infected children (group I) and twenty dummy swabs from children without gastroenteritis (group II).

The samples were collected with sterile cotton swab and processed for direct microscopic examination (gram stain, wet stain) then used culture for isolation and identification of microorganisms (swabs streaked on blood agar .macConky agar and choclate agar).

The morphology of different types of colonies was recorded and smears of these different colonies were done by study the Gram's stain reaction, microscopic characteristics, colony morphology on growth media then different types of colonies were subculture and stored for further biochemical tests to reach complete identification of each isolates, in addition some isolated of gram negative were diagnosed by API 20 E ® system (trade mark of commercial strips for identification of gram negative rods).

Results: Ninety two' bacterial isolated were obtained in this study, sixty four from group I and twenty eight from group II.

The results revealed that there is statistical significant difference (P < 0.05) between the two groups concerning E.dbfr and Klebsiella species which is the most common bacteria isolated from group I, while the most common bacteria isolated from group II was Staphylococcus epidermials.

Conclusion: There is significant appearance of E.coli and Klebsiella species in dummy sucking gastroenteritis infected children and suggested that the use of a pacifier is significant risk factor for recurrent of gastroenteritis.