Determination of the antibacterial activity of mta, and pulpotec filling materials against selected microorganisms : in vitro study

Abstract EN

Background: This in vitro study concerned with the assessment of the antibacterial effects of two materials used as a pulpotomy medicament, mineral trioxide aggregate (MTA) and a new filling material Pulpotec (PD) on various species of microorganisms, using agar diffusion test.

Materials and methods: Abase layer of petri plates was made using Muller-Hinton agar.

After solidification, two cavities were made in agar and filled with fresh mixed materials.

S/aphy/ococcus aureus.

Enterococcus Faecalis, and Escherichia coli were seeded by pour plate.

The plates were preincubated for 2 hours at room temperature followed by incubation at 37-C.

The inhibition zone diameters were measured in (mm) at 24, 48 and 72 hours.

Data were analyzed statistically using descriptive and analytic statistics, the descriptive including mean and standard deviation, and the analytic statistics in which t-test was run far multiple comparisons (between materials, and micraarganisms).

Results: The highest mean diameters of growth inhibition zones were observed around PD against all tested microorganisms over 72 hours.

According to t-test, there was a high significant difference between PD in comparison with MTA (P< 0.01).

The difference was highly significance between the tested micraarganisms.

Conclusion: The in vitro antibacterial activity of pulpotec offers additional advantage over MTA.