Gene polymorphisms of TNF-α and IL-10 related to rheumatic heart disease
Joint Authors
Tawfiq, Inas
Abd al-Salam, Manal
Abd al-Karim, Arwa
Yusri, Muna
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 11, Issue 1 (31 May. 2010), pp.33-45, 13 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2010-05-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Topics
Abstract EN
Background : Rheumatic fever (RF) is inherited as a single recessive gene.
Several genes are Likely to predispose an individual to develop rheumatic fever and rheumatic heart disease (RHD).
Polymorphisms of TNF-α gene were associated with susceptibility to develop RF.T cells from all rheumatic fever patients produce significant amounts of TNF-α in response to steptococcal peptides with the highest production attained by the chronic rheumatic heart disease patients,and IL-10 expression was characterized in heart tissue of RHD patients by immuno-histochemistry.
Objectives : To test the relation of RHD and gene polymorphisms of proinflammatory cytokines TNF-α gene at position -308 and anti–inflammatory IL-10 gene at position -1082.
Subjects and Methods : This study included 20 children with chronic rheumatic heart disease (group A) and 10 healthy children as a control group (Group B).
Patients group was classified into patients with single and multiple valvular lesions, both of them were classified according to the severity by Echocardiography into : Group I: mild valvular lesion (n=7) Group II: Moderate lesion (n=4) Group III: severe lesion (n=9) Real time PCR was done for both TNF-α at-308 and IL-10 at position – 1082.
Results : All cases showed significant higher frequency of TNF-α homozygous genotype G/G compared to control group (p <0.05,OR,11).
Cases with severe valvular lesions showed increased frequency of homozygous genotype G/G and increased frequency of IL-10 genotype G/A in cases compared to control group (p ≤0.05,OR 13).
There was no statistically significant difference in frequency of IL-10 genotypes among cases and control groups regarding to severity of valvular lesions.
Composite genotypes (TNF-α G/G,IL-10 G/A) were higher in cases compared to control group (P ≤ 0.01), while composite genotypes (TNF-α G/A,IL -10 G/G) were higher in control group compared to cases groups.
Conclusions : Susceptibility to RHD is associated with cytokine gene polymorphisms of TNF-α homozygous genotype G/G at-308 and IL-10 G/A at 1082.
Composite genotypes (TNF-α G/G,IL-10 G/A) had high risk for RHD, while composite genotypes (TNF-α G/A,IL-10 G/G) may be protective genotypes.
American Psychological Association (APA)
Tawfiq, Inas& Abd al-Salam, Manal& Abd al-Karim, Arwa& Yusri, Muna. 2010. Gene polymorphisms of TNF-α and IL-10 related to rheumatic heart disease. The Egyptian Journal of Medical Human Genetics،Vol. 11, no. 1, pp.33-45.
https://search.emarefa.net/detail/BIM-262018
Modern Language Association (MLA)
Tawfiq, Inas…[et al.]. Gene polymorphisms of TNF-α and IL-10 related to rheumatic heart disease. The Egyptian Journal of Medical Human Genetics Vol. 11, no. 1 (May. 2010), pp.33-45.
https://search.emarefa.net/detail/BIM-262018
American Medical Association (AMA)
Tawfiq, Inas& Abd al-Salam, Manal& Abd al-Karim, Arwa& Yusri, Muna. Gene polymorphisms of TNF-α and IL-10 related to rheumatic heart disease. The Egyptian Journal of Medical Human Genetics. 2010. Vol. 11, no. 1, pp.33-45.
https://search.emarefa.net/detail/BIM-262018
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 43-45
Record ID
BIM-262018