Vaccination of chickens with live Newcastle disease virus vaccines adjuvanated with Nigella sativa oil

Abstract EN

Two hundred, five-week old chickens were divided into six equal groups (30 chicks / group) and vaccinated with different live NDV vaccines in Nigella sativa oil.

The vaccinated birds were bled at one-week interval post-vaccination over six weeks and the collected sera were tested by the HI test.

Half of each group was challenged 21-days post-vaccination by intramuscular route of inoculation with 2X105EID50 of the velogenic viscerotropic Newcastle disease virus (VVNDV).

Birds were observed for 15 days post-challenge for any clinical signs of Newcastle disease.

Deaths within this period were subjected to post mortem examination.

The remaining birds in each group were observed after challenge till 42-days post-vaccination.