Studies on recent IBD virus field variant isolates : Genomic identification and differentiation using RT-PCR-RFLP
Joint Authors
Amir, M. M.
Nasif, Suad Abd al-Aziz Abd al-Wanis
Source
Beni Sueif Veterinary Medical Researches
Issue
Vol. 15, Issue 2 (31 Dec. 2005), pp.134-138, 5 p.
Publisher
Beni-Suef University Faculty of Veterinary Medicine
Publication Date
2005-12-31
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Topics
Abstract EN
In the present study, 3 pooled periventricular homogenates were collected from 3 broiler flocks, of chicken 15 to 30 days old, from Monomial Governorate.
The 3 flocks were suffered from low growth rate, poor feed conversion rate, uneven growth and increased mortalities.
Necropsy of dead chickens revealed proventriculitis with increased proventriculus size.
IBD viral antigen was detected in the pooled periventricular homogenate of each flock by AGPT using reference antibodies against IBDV and RT-PCR technique.
No other viruses were detected; such as Reo virus, CAV, NDV, IBV and ALV-J.
Further characterization of the IBDV isolates were conducted by RFLP assay on PCR products using MboI and BstOI restriction enzymes.
Results demonstrate that the 3 IBDV isolates are identical in their RFLP pattern and related to the Del / E variant strain of IBDV.
American Psychological Association (APA)
Amir, M. M.& Nasif, Suad Abd al-Aziz Abd al-Wanis. 2005. Studies on recent IBD virus field variant isolates : Genomic identification and differentiation using RT-PCR-RFLP. Beni Sueif Veterinary Medical Researches،Vol. 15, no. 2, pp.134-138.
https://search.emarefa.net/detail/BIM-26209
Modern Language Association (MLA)
Amir, M. M.& Nasif, Suad Abd al-Aziz Abd al-Wanis. Studies on recent IBD virus field variant isolates : Genomic identification and differentiation using RT-PCR-RFLP. Beni Sueif Veterinary Medical Researches Vol. 15, no. 2 (2005), pp.134-138.
https://search.emarefa.net/detail/BIM-26209
American Medical Association (AMA)
Amir, M. M.& Nasif, Suad Abd al-Aziz Abd al-Wanis. Studies on recent IBD virus field variant isolates : Genomic identification and differentiation using RT-PCR-RFLP. Beni Sueif Veterinary Medical Researches. 2005. Vol. 15, no. 2, pp.134-138.
https://search.emarefa.net/detail/BIM-26209
Data Type
Journal Articles
Language
English
Notes
28
Record ID
BIM-26209