Preparation of diagnostic lysate antigens for rapid evaluation of local entero-3 vaccine

Abstract EN

Studies were conducted to determine the utility of lysate antigens for rapid evaluation of the local entero-3 vaccine ; antigens were prepared from cell cultures infected with bovine Rota virus (BRV) and bovine corona virus (BCV) as well as from Enter toxigenic E.

coli strain K99.

Prepared antigens were tested with field serum samples collected from both late pregnant entero-3 vaccinated cows and their offspring's using different serological assays including : micro agglutination test, indirect ELISA and immunofluorescent antibody technique.

Results of this endeavor were correlated to that of the standard virus neutralization test.

The locally prepared antigens were proved useful for vaccine evaluation.

Moreover, these antigens are recommended for both detection and assessment post vaccination or post infection of sera-conversion against BRV, BCV and E.

coli.