Detection of bovine herpesvirus type 1 and bovine ephemeral fever virus antigens in cattle lymph nodes using three immunological assays
Joint Authors
Abd al-Munim, A. S.
Tamam, Sabri Muhammad
Source
Beni Sueif Veterinary Medical Researches
Issue
Vol. 15, Issue 2 (31 Dec. 2005), pp.203-207, 5 p.
Publisher
Beni-Suef University Faculty of Veterinary Medicine
Publication Date
2005-12-31
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Topics
Abstract EN
Lymph nodes (Ln) are the preferred samples for virus isolation and detection.
In the present study, carcass and visceral Ln of apparently healthy cattle were screened for the presence of bovine herpes virus type 1 (BHV-1) and bovine ephemeral fever virus (BEFV) antigens.
A total of 198 Ln (114 carcasses Ln and 84 visceral Ln) were collected.
Lymph node homogenates were assayed by agar gel precipitation test (AGPT), rapid Staphylococcal protein A (SPA) agglutination test and Dot-ELISA.
The overall results revealed that BHV-1 antigens were detected in 43.9%, 56.1 % and in 68.4 %.
Of carcass Ln, and in 29.8 %, 47.6 % and 57.1 % of visceral Ln collected from slaughtered cattle by AGPT, SPA agglutination test and Dot-ELISA respectively.
On the other hand, BEFV antigens were detected in 5.3 %, 38.6 % and 52.6 % of carcass Ln, and in 6 %, 41.7 % and 54.8 % of visceral Ln collected from slaughtered animals by AGPT, rapid SPA agglutination test and Dot-ELISA respectively.
The results showed high percentage of positive samples with SPA agglutination test and Dot-ELISA in comparison to AGPT for both BHV-1 and BEF.
American Psychological Association (APA)
Tamam, Sabri Muhammad& Abd al-Munim, A. S.. 2005. Detection of bovine herpesvirus type 1 and bovine ephemeral fever virus antigens in cattle lymph nodes using three immunological assays. Beni Sueif Veterinary Medical Researches،Vol. 15, no. 2, pp.203-207.
https://search.emarefa.net/detail/BIM-26310
Modern Language Association (MLA)
Tamam, Sabri Muhammad& Abd al-Munim, A. S.. Detection of bovine herpesvirus type 1 and bovine ephemeral fever virus antigens in cattle lymph nodes using three immunological assays. Beni Sueif Veterinary Medical Researches Vol. 15, no. 2 (2005), pp.203-207.
https://search.emarefa.net/detail/BIM-26310
American Medical Association (AMA)
Tamam, Sabri Muhammad& Abd al-Munim, A. S.. Detection of bovine herpesvirus type 1 and bovine ephemeral fever virus antigens in cattle lymph nodes using three immunological assays. Beni Sueif Veterinary Medical Researches. 2005. Vol. 15, no. 2, pp.203-207.
https://search.emarefa.net/detail/BIM-26310
Data Type
Journal Articles
Language
English
Notes
36
Record ID
BIM-26310