Preparation of inactivated tissue culture rabies vaccine adjuvanted with natural immune stimulants

Abstract EN

The challenge virus standard (CVS-11) strain of fixed rabies virus was propagated in weaning mice brains and in suckling mice brains.

It was also propagated on baby hamster kidney cell line (BHK-21) with use of diethylaminoethyl-dextran (DEAEDextran).

These passages were titrated in 3-4 weeks old white Swiss mice using mouse inoculation test (MIT).

The virus harvest was concentrated using zinc acetate method, inactivated by beta-propiolactone (BPL) and adjuvant with combination of vitamin E and selenium.

The prepared adjuvant inactivated tissue culture rabies vaccine was subjected to quality control tests as safety, sterility and potency using National Institute of Health (NIH) test against reference vaccine.