Effect of scaling and root planning with topical doxycycline versus scaling and root planning only on the subgingival microbiota of chronic periodontitis patients

Abstract EN

The objective of the present investigation is to compare the effect of topical application of Doxycycline 10% (AtridoxTM) following scaling and root planning, thus, versus scaling and root planning alone on the subgingival plaque composition of patients with generalized severe chronic periodontitis. Using split mouth design, subgingival plaque samples were taken at the baseline and 2 months after treatment.

Microbiological analysis was carried out using checkerboard DNA probe technology.

Plaque samples were screened for the presence of Actinomyces naeslundii, Tannerella forsythia, Campylobacter conscisus, Campylobacter curva, Campylobacter rectus, Capnocytophaga sputigena, Eikenella corrodens, Fusobacterium nucleatum ss vincentii, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Streptococcus oralis, Treponema denticola, and Aggregatibacter actinomycetemcomitans serotype b.

After two months posttreatment, there was a significant decrease in total bacterial counts in both groups.

Pathogens such as Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola were reduced significantly when AtridoxTM was used in addition to scaling and root planning, while Actinomyces naeslundii and Streptococcus oralis levels were increased.

The topical application of Doxycycline 10% as an adjunct to scaling and root planning is beneficial to the non-surgical management of patients with generalized severe chronic periodontitis.