Genetic diversity analysis of fragrantissima (forskal)‎ schultz bip populations collected from different regions of Jordan using RAPD markers

Abstract EN

Medicinal plants are still used traditionally to cure some illnesses.

Achilles fragrantissima is among medicinal species used by rural communities in Jordan.

The genetic diversity of this species was studied using Random Amplified Polymorphic DNA (RAPD) markers of five populations sampled from five locations in Jordan.

Fifteen polymorphic primers were used for the construction of the dendrogram and the similarity matrix.

A total of 2599 bands were obtained, 420 of them were polymorphic.

Similarity values between the populations samples ranged from 0.58 between the samples of Madera (1 population) to 0.02 between Shoeblack (3 populations) and Mwaqqar (2 populations).

High similarity values were also recorded between Malabar (1population) and Marfa (1 population), two samples of Ma'am (3), two samples of Madera (1), two samples of Mwaqqar, Marfa (1), and Madera (1) populations with values from 0.54 to 0.49, respectively.

Four main clusters were obtained based on UPGMA dendrogram.

Both Mwaqqar2 and Ma'am 3 populations formed separate sub-clusters.

This emphasizes the presence of genetic diversity among the studied populations.

RAPD analysis has the ability to discriminate between Achilles fragrantissima populations.