Isolation of caga and vaca genes from H. pylori infected patients with various gastroduodenal lesions

Abstract EN

This study aimed to learn the incidence of Helicobacter pylon infection in patients with various gastroduodenal endoscopic lesions and the frequency of virulence H.pylori associated genes CagA and VacA in these patients One hundred seventy six patients (96 males and 80 females) attending endoscopy units for various dyspeptic symptoms were studied Antral biopsies were obtained to detect H.pylon by rapid urease test, culturing and histopathology examination Twenty five patients with positive H pylon isolates who were found to be mannose resistant, were tested for cytotoxic.

associated (CagA) and vacuolating cytotoxin A (VacA) genes.

Among studied patients positive H.pylori detected by rapid urease test culturing and histopathology examination (from 50 patients only) were 113 (63%).

127 (71%) and 25 (50%) respectively Out of 25 patients with positive H.pylori isolates who were found to be mannose resistant, positive genes of either CagA or VacA were detected in 18 (72%) patients with positive isolates, while positivity of both genes were detected in 13(52%) patients with positive isolates.

Five (45.4%) and 5 (45.4%) out of patients with duodenal ulcers and gastritis respectively were positive for both (CagA) and (VacA) genes In conclusion, the highest detection rate of H.pyloti infection was by bacterial culture A correlation between CagA and VacA genes and endoscopic lesions of duodenal ulcers and gastritis was found