Therapeutic effect of vitamin c in endotoxin-induced metabolic acidosis and oxidative stress in lambs

Abstract EN

Fcndotoxemia associated with Gram-negative sepsis is an emerecncv clinical_ state that causes rapid biochemical chants and substantial mortalities in livestock.

The present stud v aimed" to investigate the therapeutic effect of vitamin C on blood gases, acid-base balance and nuns oxide in Iamb model of lipopolysaccharide [LPS1, induced end toxemia.

The study was conducted on 15 lambs, 3-5 months of a J Animals were randomly grouped into 3 groups, five each as following Group 1 (control) : received only normal saline, Group 2 : received only LPS at dose 25 ug / kg B.W.

and Group 3 ; received vitamin C at dose 4 mg / kg B.W.

one hour before LPS infusion.

Saline (10 ml) or I PS "n saline were infused through jugular cannula.

Inc.

experimental 'fever elicited by IV injection of LPS 25 ug / kg / B.W.

was accompanied by metabolic acidosis, hypoxemia and significance increased production of nitric oxide.

A significant decrease in blood pH.

Base excess and HCO-" was noticed during fever.

Although the concentrations of I ICO- were decreasing till the end of the experiment, there was no substantiaffalls in PC02 for compensation of the noticed acidosis.

However, hypoxemia was evident as indicated with marked reduction in oxen saturation and oxygen tension.

Nitric oxide level increased during the period of experiment and reached peak 5 hours post I.l > Sr administration 1 retreatment with i.v.

20 mg / kg B.W.

of vitamin C an hour pre I PS changes m pH, HC03 and nitric oxide.

Possible mechanisms bv high vitamin C could affect the LPS-induced disturbances of acid-base balance are being considered.

Finally, it could be concluded that vitamin C pretreatment prevented LPS -induced metabolic acidosis and nitric oxide production.