Quantification and stability evaluation of the highly specific angiotensin-converting enzyme (ACE) Inhibitor captopril in human plasma using a gas chromatographic method with N,N,N',N'-tetramethyl-2-butenediamide derivatizing agent
Joint Authors
Abu Shandi, Khalid Hamad
Redel, Engelbert
Source
Issue
Vol. 4, Issue 2 (30 Jun. 2009), pp.183-194, 12 p.
Publisher
Yarmouk University Deanship of Research and Graduate Studies
Publication Date
2009-06-30
Country of Publication
Jordan
No. of Pages
12
Main Subjects
Topics
Abstract EN
An enhanced and sensitive GC–ECD assay is presented for the highly specific angiotensin-converting enzyme (ACE) inhibitor, Captopril.
This method provides an improved sensitivity over other previously published assays of Captopril derivative.
The method involves derivatization with N, N, N', N'-tetramethyl-2-butenediamide and formation of its methyl ester followed by a rapid extraction technique by using acetonitrile as deproteinizing agent.
Enalapril was used as internal standard, and XE-60-S-valine-S-phenylethylamide (Chrompack), 25 m x 0.25 mm I.D.
and 0.12 pm film thickness was used as the stationary phase in the capillary GC column.
Captopril was measured as the methyl ester of its N, N, N', N'-tetramethyl-2- butenediamide derivative.
The method was fully validated according to United States Food and Drug Administration requirements (linearity, precision, trueness, quantification limit, detection limit, recovery, stability and specificity).
The assay was linear from 0.010 to 2.0 μg / ml with a mean recovery of 99%.
American Psychological Association (APA)
Abu Shandi, Khalid Hamad& Redel, Engelbert. 2009. Quantification and stability evaluation of the highly specific angiotensin-converting enzyme (ACE) Inhibitor captopril in human plasma using a gas chromatographic method with N,N,N',N'-tetramethyl-2-butenediamide derivatizing agent. Jordan Journal of Chemistry،Vol. 4, no. 2, pp.183-194.
https://search.emarefa.net/detail/BIM-266477
Modern Language Association (MLA)
Abu Shandi, Khalid Hamad& Redel, Engelbert. Quantification and stability evaluation of the highly specific angiotensin-converting enzyme (ACE) Inhibitor captopril in human plasma using a gas chromatographic method with N,N,N',N'-tetramethyl-2-butenediamide derivatizing agent. Jordan Journal of Chemistry Vol. 4, no. 2 (Jun. 2009), pp.183-194.
https://search.emarefa.net/detail/BIM-266477
American Medical Association (AMA)
Abu Shandi, Khalid Hamad& Redel, Engelbert. Quantification and stability evaluation of the highly specific angiotensin-converting enzyme (ACE) Inhibitor captopril in human plasma using a gas chromatographic method with N,N,N',N'-tetramethyl-2-butenediamide derivatizing agent. Jordan Journal of Chemistry. 2009. Vol. 4, no. 2, pp.183-194.
https://search.emarefa.net/detail/BIM-266477
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 193-194
Record ID
BIM-266477