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Species sensitive electrochemical method for enrichment of azadirachtin-a from neem seed
Joint Authors
Source
Issue
Vol. 4, Issue 3 (30 Sep. 2009), pp.271-278, 8 p.
Publisher
Yarmouk University Deanship of Research and Graduate Studies
Publication Date
2009-09-30
Country of Publication
Jordan
No. of Pages
8
Main Subjects
Abstract EN
Azadirachtin the active principle of Azadirachta-indica J.
Juss.
(Neem) ranges from 0.3 to0.6 percent.
A simplified procedure, when compared to established methods, has been developed to enrich azadirachtin-A to 50 percent using minimum possible steps without resorting to chromatographic techniques.
It involves additional partition with CCl4 before final extraction which removes most of nimbin, salanin and allied triterpenoids.
Enriched product finds immense utility in preparing formulations with high azadirachtin concentration.
DCP and DPP polarogrphic methods have been developed for the qualitative as well as quantitative analysis of azadirachtin in plant product and its formulation (Neemgold®).
Azadirachtin produces a well-defined polarographic wave / peak in 0.1M Tetraethyl ammonium bromide at pH 3.3 ± 0.1 with E½ / Ep= -1.48V/SCE.
The wave/peak height is found to be proportional to the concentration of Azadirachtin.
The developed procedure was used for the analysis of Azadirachtin in extract of Azadirachtin indica (seeds) and market formulation.
Statistical treatment of the observed voltammetric data revealed high accuracy and good precision of determination.
American Psychological Association (APA)
Thakur, Kirti& Pitre, K. S.. 2009. Species sensitive electrochemical method for enrichment of azadirachtin-a from neem seed. Jordan Journal of Chemistry،Vol. 4, no. 3, pp.271-278.
https://search.emarefa.net/detail/BIM-266484
Modern Language Association (MLA)
Thakur, Kirti& Pitre, K. S.. Species sensitive electrochemical method for enrichment of azadirachtin-a from neem seed. Jordan Journal of Chemistry Vol. 4, no. 3 (Sep. 2009), pp.271-278.
https://search.emarefa.net/detail/BIM-266484
American Medical Association (AMA)
Thakur, Kirti& Pitre, K. S.. Species sensitive electrochemical method for enrichment of azadirachtin-a from neem seed. Jordan Journal of Chemistry. 2009. Vol. 4, no. 3, pp.271-278.
https://search.emarefa.net/detail/BIM-266484
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 278
Record ID
BIM-266484