Polymorphism of protein fractions as biochemical markers for identification of wheat varieties

Abstract EN

The electrophoretic banding patterns of glia din, albumin and globulin in wheat varieties grown in Sulaimanyah were determined by acid-polyacrylamide gel electrophoresis (Acid-PAGE) and Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

The varieties of bread wheat used in the study showed 21 different patterns : six of them were identified to be corresponding to Ω-glia din, three to the γ-glia din, six to the β-glia din, and other six to the α-glia din.

The glia din patterns of wheat bread varieties greatly differed from the patterns of Italian wheat variety (Contention), and the variation detected in wheat varieties from Sulaimanyah was limited to forty seven patterns.

Only Tammuz showed the presence of gamma 47.

The glia din gamma 47 is only genetic markers for technological properties in wheat.

The electrophoretic banding patterns showed variations between the bread wheat varieties.

The highest value of similarity was observed between the varieties Cham 4 and Cham 6 for glia din analysis.

The glia din pattern revealed more polymorphisms than the albumin and globulin pattern.

On the other hand, in durum wheat varieties, two types of omega glia din: omega glia din 33 and 35 and two types of gamma glia din were found: gamma glia din 42 and gamma glia din 45.

A total of 84 band patterns were identified (durum wheat), of which 2 different mobility bands were in the region of omega glia dins, 1 in the region of gamma glia dins, 2 in the region of beta glia din and 2 in the region of alpha glia dins.

Genetic diversity index was highest in Cress Kurd and followed by Ovate.