Genetic relatedness among wild and cultivated almond genotypes using randomly amplified polymorphic DNA (RAPD) markers in Jordan
Joint Authors
al-Rawashidah, Ibrahim Muhammad
al-Ghazzawi, Abd al-Latif Ali
al-Tawaha, Abd al-Rahman M.
Source
Jordan Journal of Biological Sciences
Issue
Vol. 2, Issue 2 (30 Jun. 2009), pp.89-96, 8 p.
Publisher
The Hashemite University Deanship of Academic Research and Graduate
Publication Date
2009-06-30
Country of Publication
Jordan
No. of Pages
8
Main Subjects
Topics
Abstract AR
استخدمت تقنية RAPD لدراسة الارتباط الوراثي بين 16 صنف لوز المزروع في الحقل الوراثي (محطة المشقر) و الأنواع البرية و المزروعة في حقول المزارعين.
خمسة بادئات أظهرت باندات متشبعة استخدمت لبناء الشجرة العنقودية و التشابه.
قيم التشابه بين الأنواع المدروسة كانت بين صفر-50 %.
أعلى قيم التشابه كانت بين نوعين من صنف اللوز فوسكي (50 %) و تونو 48 %.
إن التحليل RAPD أثبت وجود اختلافات وراثية بين الأنواع المدروسة.
صنف عوجا كان أكثر بعدا وراثيا مقارنة الأصناف الأخرى و كانت الأنواع البرية و الأنواع الحلوة تجمعت في مجموعات مفصولة عن بعضهم.
إن استخدام عدد قليل من العينات مع أكثر البادئات المتشبعة تعبر المفتاح للحصول على نتائج للعلاقات الوراثية بين أنواع اللوز.
Abstract EN
Randomly amplified polymorphic DNA (RAPD) technique was used to study the genetic relatedness between 16 almond cultivars (Prunus.
Dulcis) grown at field gene bank (Al-M'shaqer Research Station), wild genotypes and farmers' orchards genotypes in Jordan.
Five primers showed polymorphic bands were used for construction dendrogram and similarity matrix.
Similarity values among the studied genotypes were ranged from 0.000 to 0.500.
High similarity values were obtained between the two sweet almond cultivars Fuksii (0.500) and Tuono (0.480).
RAPD analysis confirmed the existence of genetic variation among tested almond cultivars.
Oja cultivar was the most distant compared to the rest of the cultivars, even, wild types and sweet genotypes were grouped separately.
Using a minimum number of samples with the most polymorphic RAPD primers was a key for having rapid results of genetic relationships among almond genotypes.
American Psychological Association (APA)
al-Ghazzawi, Abd al-Latif Ali& al-Rawashidah, Ibrahim Muhammad& al-Tawaha, Abd al-Rahman M.. 2009. Genetic relatedness among wild and cultivated almond genotypes using randomly amplified polymorphic DNA (RAPD) markers in Jordan. Jordan Journal of Biological Sciences،Vol. 2, no. 2, pp.89-96.
https://search.emarefa.net/detail/BIM-267006
Modern Language Association (MLA)
al-Ghazzawi, Abd al-Latif Ali…[et al.]. Genetic relatedness among wild and cultivated almond genotypes using randomly amplified polymorphic DNA (RAPD) markers in Jordan. Jordan Journal of Biological Sciences Vol. 2, no. 2 (Jun. 2009), pp.89-96.
https://search.emarefa.net/detail/BIM-267006
American Medical Association (AMA)
al-Ghazzawi, Abd al-Latif Ali& al-Rawashidah, Ibrahim Muhammad& al-Tawaha, Abd al-Rahman M.. Genetic relatedness among wild and cultivated almond genotypes using randomly amplified polymorphic DNA (RAPD) markers in Jordan. Jordan Journal of Biological Sciences. 2009. Vol. 2, no. 2, pp.89-96.
https://search.emarefa.net/detail/BIM-267006
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 95-96
Record ID
BIM-267006
