Epidemiological aspect of bovine viral diarrhea virus-ii infection in dairy Cattle

Abstract EN

The purpose of this study was to characterize the clinical and virology features of the mucosal and thrombocytopenic BVDV infection in infected dairy cattle.

Strategy of examination included clinical examination of diseased animals, serum samples (n = 31) represented all diseased cattle, milk samples (n^) represented clinically diseased cattle and vaginal swabs (n = 8) from the hemorrhagic diseased and aborted cattle were tested.

An international reference strain (NADL: National Animal Disease Laboratory) and local cytopathic BVDV genotype-II strain (Becerra-CP 58 / 99) were used as positive controls.

The virus was isolated from the 8 vaginal swabs and 5 out of the 8 milk samples.

All the isolates were ncp that no CPEs were noticed over the 3 passages.

By specific BVDV genotype -II monoclonal antibodies (MAbs) against gp53, the viral antigen was identified using Fluorescence isothiocyanate (FITC)-conjugated anti-bovine IgG (specific intra cytoplasmic fluoresce granules) and horseradish peroxidase (HRP)-conjugated anti-bovine IgG (specific intra cytoplasmic brown granules) were detected.

By enzyme linked immunosorbant assay (ELISA) technique, all serum samples were positive against the BVDV that had neutralizing antibodies titer ranges from > 1 / 128 to > 1 / 512.

In conclusion, BVDV type-II do exist in cattle population and the understanding the molecular epidemiology is fundamental.

Improved diagnostic and control strategies are essential to reduce losses inflected by BVDVs infection.