Pathological effect of acrylamide on the reproductive performance of the male rabbit

Abstract EN

Acrylamide caused significant increases in sperm head abnormalities, decreased entry of sperm to uterus and consequently decreased in the fertility rate.

Therefore, present study aimed at evaluating the histopathology adverse effects of toxicity of acrylamide on the male genital organs with special reference to semen quality.

Male rabbit (buck) were classified into 2 groups.

Group I consisted of sixteen rabbits which were daily intubated orally with acrylamide soluble in water, given with stomach tube until the end of the experiment.

Group II consisted of 4 animals and they were kept as a control.

The daily dose was 63 mg acrylamide / Kg body weight up to 8 weeks-the whole length of spermatogenic cycle in rabbit.

Four rabbits were sacrificed each two weeks, from intoxicated group, while 2 rabbits were sacrificed on the 2nd week and at 8th weeks from the control group.

Semen was collected twice weekly using a teaser and an artificial vagina.

The semen samples were assessed immediately after collection for, individual motility, sperm abnormality, sperm cell concentration and acrosome integrity.

The present results revealed that, acrylamide treatment resulted in severe testicular lesions giving the picture of testicular atrophy; the seminiferous tubules showed severe and over spread interstitial edema and prominent testicular degeneration.

Acrylamide treatment resulted in a significant reduction in the sperm individual motility, total sperm abnormalities and the sperm cell concentration.

Meanwhile, the percentage of the dead sperm and the total sperm abnormalities were significantly increased.

Finally, it could be concluded that oral daily administration of 63 mg / kg B.W acrylamide to the male rabbit up to 8 weeks has a generalized deleterious effect, particularly on fertility parameters as evidenced from semen evaluation and the associated testicular degeneration.