Changes in serum levels of soluble interleukin-2 receptor and fas–ligand expression in relation to disease activity of systemic lupus erythematosus
Other Title(s)
العلاقة بين التغيرات التي تحدث في معدل مستقبلات انترليوكين الذائبة في الدم و كذلك ظهور فاز- ال علي خلايا الدم و علاقتهم بالنشاط المرضي للذئبة الحمراء
Joint Authors
Jawdah, al-Sayyid Ahmad
al-Harun, Ahmad Shukri
Husni, Ahmad
Abd al-Karim, Muhammad Isamil
Ahmad, Muhammad Shees
Fuda, Muhammad
Source
Egyptian Rheumatology and Rehabilitation
Issue
Vol. 35, Issue 3 (31 Jul. 2008), pp.327-336, 10 p.
Publisher
The Egyptian Society for Rheumatology and Rehabilitation
Publication Date
2008-07-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract AR
طريقة البحث : أجريت هذه الدراسة على 36 مريض بالذئبة الحمراء و كذلك على 25 من الأصحاء كمجموعة ضابطة، و تم جمع عينات من الدم الوريدي للمجموعتين.
النتائج : أثبتت الدراسة بأن معدل مستقبلات الأنترلوكين 2 الذائبة بالدم تزيد بنسبة ذات دلالة إحصائية عن المجموعة الضابطة (P < 0.001).
و كانت تلك الزيادة أيضا بقيمة ذات دلالة إحصائية في المرضى الذين يعانون من الإرهاق و إصابة الكلى و الرئتين.
و كذلك أثبتت الدراسة بأن ظهور الفاز–ال على خلايا الدم الطرفية للمرضى بنسبة 88.9 %.
و لم تظهر في المجموعة الضابطة.
و كانت نسبة حدوثها أعلى في المرضى الذين يعانون من الإرهاق و إصابة الجلد و الكلى.
و بمقارنة هذه النتائج لمرضى الذئبة الحمراء بالعلامات الأخرى المعروفة لهذا المرض وجد أنها ذات قيمة إحصائية توازي بداية و شدة المرض.
الاستنتاج : و عليه يمكن اعتبار SilL2R دليل على بداية النشاط المرضي و Fas-L دليل على حدة المرض.
Abstract EN
Objective : Serum soluble IL-2 receptors (sIL2R) levels and Fas-ligand (Fas-L) expression on peripheral blood mononuclear cells (PBMC) were determined in patients with Systemic Lupus Erythematosus (SLE) to assess whether there was any relationship between them and disease activity.
Methodology : Enzyme Linked Immunosorbant assay (ELISA) technique was done on serum samples collected from 36 SLE patients and 25 healthy controls for determination of sIL2R level.
RT-PCR was done also on PBMC samples collected from the same patients and controls for detection of Fas-L mRNA.
Results : We found significant increase of sIL2R in the SLE group (327.6 ±73.5 pg / ml) compared to healthy controls (119.7 ± 12.6 pg / ml) (p < 0.001).
Levels of sIL2R were found to correlate significantly with clinical manifestation and serological markers of active SLE: fatigue (p < 0.05), renal involvement (p < 0.01), pulmonary involvement (p < 0.05), high levels of anti-ds DNA antibody (p < 0.001) and high C3 level (p < 0.0001).
Fas-L mRNA was expressed in PBMC from (88.9%) SLE patients and not detected in healthy controls.
Fas-L positive SLE patients correlate significantly with clinical manifestation and serological markers of active SLE: fatigue (p < 0.0001), rash (p < 0.05), renal affection (p < 0.001), high levels of anti-ds DNA antibody (p < 0.0001) and high C3 level (p < 0.0001).
Levels of sIL2R and Fas-L expression correlate significantly with disease activity (p<0.001, 0.001, 0.05, 0.005, respectively).
Conclusion : these findings indicate that sIL2R represent a new early useful serological marker to monitor disease activity in SLE patients.
Fas-L expression increased in SLE patients, this increasing was in parallel to disease activity, so it is used as late marker to monitor the severity of the disease.
American Psychological Association (APA)
Jawdah, al-Sayyid Ahmad& Ahmad, Muhammad Shees& al-Harun, Ahmad Shukri& Fuda, Muhammad& Abd al-Karim, Muhammad Isamil& Husni, Ahmad. 2008. Changes in serum levels of soluble interleukin-2 receptor and fas–ligand expression in relation to disease activity of systemic lupus erythematosus. Egyptian Rheumatology and Rehabilitation،Vol. 35, no. 3, pp.327-336.
https://search.emarefa.net/detail/BIM-26829
Modern Language Association (MLA)
Jawdah, al-Sayyid Ahmad…[et al.]. Changes in serum levels of soluble interleukin-2 receptor and fas–ligand expression in relation to disease activity of systemic lupus erythematosus. Egyptian Rheumatology and Rehabilitation Vol. 35, no. 3 (Jul. 2008), pp.327-336.
https://search.emarefa.net/detail/BIM-26829
American Medical Association (AMA)
Jawdah, al-Sayyid Ahmad& Ahmad, Muhammad Shees& al-Harun, Ahmad Shukri& Fuda, Muhammad& Abd al-Karim, Muhammad Isamil& Husni, Ahmad. Changes in serum levels of soluble interleukin-2 receptor and fas–ligand expression in relation to disease activity of systemic lupus erythematosus. Egyptian Rheumatology and Rehabilitation. 2008. Vol. 35, no. 3, pp.327-336.
https://search.emarefa.net/detail/BIM-26829
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 335-336
Record ID
BIM-26829