Does injection of metanephric mesenchymal cells improve renal function in rats?
Joint Authors
Yi, Zhe Wen
Jiao, Yu-qing
He, Xiao-jie
Liu, Xi-hong
He, Qing-nan
Huang, Dan-lin
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 22, Issue 3 (30 Jun. 2011), pp.501-510, 10 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2011-06-30
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Topics
- Kidneys
- Diseases
- Laboratory animals
- Rats
- Penal functions
- Medical examinations
- Antineoplastic drugs
- Therapeutics
- Kidney failure
- Mesenchymal stem cells
- Stem cells
Abstract EN
Chronic kidney disease (CKD) is a massive global health-care problem.
Cell therapy offers a potential treatment for CKD.
The aim of this study was to investigate whether the administration of a population of stem cells could be used to treat Adriamycin (ADR)-induced glomerulopathy in rats, a form of CKD.
We intravenously transplanted met nephric mesenchyme cells (MMCs) into rats treated with ADR.
We also induced MMC differentiation in vitro using a medium derived from serum and homogenates of ADR-induced glomerulopathy rats.
We detected the induction of an early epithelial phenotype (cytokeratin-18 expression) and a proximal tubule phenotype (vitamin D receptor expression) in vitro, and MMC-derived epithelial cells corresponding to the proximal tubule and glomeruli in vivo.
Transplantation of MMCs after induction of glomerulopathy significantly increased the cretonne clearance rate (Ccr), a marker for glomerular filtration rate, but had no significant effect on other parameters (24-hour urinary protein excretion, serum albumin, and total cholesterol).
In addition, there was no significant difference in blood urea nitrogen or serum cretonne levels in rats with and without ADR administration.
Our results indicate that MMCs might survive, engraft and differentiate into renal epithelia in vivo when transplanted into ADR-treated rats.
However, further studies are needed to determine whether MMC transplantation improves renal function and causes renal repair in this model.
American Psychological Association (APA)
Jiao, Yu-qing& Yi, Zhe Wen& He, Xiao-jie& Liu, Xi-hong& He, Qing-nan& Huang, Dan-lin. 2011. Does injection of metanephric mesenchymal cells improve renal function in rats?. Saudi Journal of Kidney Diseases and Transplantation،Vol. 22, no. 3, pp.501-510.
https://search.emarefa.net/detail/BIM-268345
Modern Language Association (MLA)
Jiao, Yu-qing…[et al.]. Does injection of metanephric mesenchymal cells improve renal function in rats?. Saudi Journal of Kidney Diseases and Transplantation Vol. 22, no. 3 (Jun. 2011), pp.501-510.
https://search.emarefa.net/detail/BIM-268345
American Medical Association (AMA)
Jiao, Yu-qing& Yi, Zhe Wen& He, Xiao-jie& Liu, Xi-hong& He, Qing-nan& Huang, Dan-lin. Does injection of metanephric mesenchymal cells improve renal function in rats?. Saudi Journal of Kidney Diseases and Transplantation. 2011. Vol. 22, no. 3, pp.501-510.
https://search.emarefa.net/detail/BIM-268345
Data Type
Journal Articles
Language
English
Notes
33
Record ID
BIM-268345