Development of rapid and simple analytical method for some proton pump inhibitors (PPIs)‎ using HPLC

Abstract EN

A simple, selective and rapid reversed phase High Performance Liquid Chromatographic (HPLC) method for the analysis of Pantoprazole (LNS), Omeprazole (OMP) and Esomeprazole (ESOMP) has been developed and validated.

HPLC-UV detector equipped with RP-C18 column, mobile phase Water: Acetonitrile: TEA, 60: 40) 00.5 V / V / V Ph = 7 adjusted with phosphoric acid) and a flow rate of 1ml / min were used for the quantification for each compound.

The analytical method parameter was the same except for the wave lengths; they were 285, 280.

303 nm; and the retention times were 7 min, 4.5 min and 5 min for LNS, OMP and ESOMP, respectively.

Method validation was performed for each compound, and all the parameters were acceptable.

Therefore, the method could be used as a simple, rapid and efficient option for the analysis of LNS, OMP, and ESOMP during routine tests in pharmaceutical industries because all the parameters (mobile phase, column, flow rate) of the method are the same except for the wave length which should be changed for each compound, and the change of the wave length does not need more than 2 minutes.