Simultaneous determination of amlodipine besylate and valsartan in tablets by high performance liquid chromatography with UV detection

Abstract EN

A simple, precise, accurate and sensitive high-performance liquid chromatography method with UV detection at 220 nm was developed for the simultaneous determination of Amlodipine and Valsartan drugs in pharmaceutical formulations.

The present method presents a narrow range (19.6 - 78.4 (for Amlodipine) and 32-128 (for Valsartan) μg / ml) of calibration curve and sensitivity.

Isocratic separation was employed on a C18 column (4.6 X 520 mm i.d., 5 μm) at ambient temperature.

The mobile phase consisted of water, acetonitrile and glacial acetic acid (300:700:1 by volume).

The Drugs under investigation were found to be 93 – 101 % recovery of their label claim in pharmaceutical formulations.

The separation was completed within 6 minutes.

The calibration curve was linear over the range of 19.6 – 78.4 μg / mL for Amlodipine and over the range of 32 to 128 μg / mL for Valsartan.

The Limits of Quantification (LOQ) were chosen to be the lowest concentrations in the calibration curve (19.6 μg / mL) for Amlodipine and (32 μg / mL) for Valsartan.

The Limits of Detection (LOD) were 10 μg / mL and 16 μg / mL for Amlodipine and Valsartan, respectively.

Good method precision was demonstrated for the analysis with a coefficient of variation of 0.0049 and 0.002 for amlodipine and valsartan, respectively).