Ocular changes in gardiasis : human and experimental sudies

Abstract EN

Background / Aim : cutler chugs associated with giardiasis were recorded in a number of studies.

The aim of the present study was to evaluate the frequency of ocular manifestations in children with giardiasis in relation to ages and severity of infection In addition ; the mechanisms underlying the ocular lesions were investigated through an experimental airmail study.

Subjects & Methods : the human study comprised 73 children (from 3 to 14 years old) attending the Outpatient Clinic of Pediatric and Tropical Medicine Departments of Tanta University Hospital Trey have been diagnosed as being infected with Gar cHa labia through stool examination and indirect immure fluorescence assay or scoring the severity of infection The present study also included a control group comprised 25 age-matched children All the children were sent to the Ophthalmology Department of Tanta University Hospital to undergo an ophthalmic examination of ocular motility, slit lamp examination of tie anterior clamber, direct and indirect ophthalmoscopy examination of the undue after induction of mydriasis.

In the experimental study, 90 laboratory-bred mice were used, 70 mice were infected with Gardaí labia cysts while the remaining 20 mice were kept uninfected.

The animals were divided into four groups : Group I (35 immunocompetent and infected), group E (35 immurDsuppressed and infected), group EI (10 immurocorrpetent control) and group IV (10 immunosuppressed control).

Half the number of mice was sacrificed 2 weeks post-infection (P.I) and the remaining roster was sacrificed 4 weeks PI.

At each period of examination 1- Duodenum and upper parts of jejunum of each sacrificed mouse were removed and subjected to histopathologically examination 2- Both eyes of each sacrificed mouse were enucleated and subjected to both histopathologically examination and indirect immunofluorescence assay as a trial to detect tie parasite or its antigen in the eye tissues.

Results : Tie results of human study revealed changes in tie retinal epithelium compatible with a salt and pepper appearance in 9 / 73 (123 %) children The presence of ocular changes was related to the surety of infection were the higher percentage of cases (77.8 %) was from group C (severe infection).

Also, it was observed that the children with retinal changes were significantly younger (mean age 3.8 + 0.04 years) than those without lesions.

In the experimental study, 2 weeks PI, neither histopathologically changes roar immunofluorescent deposits were detected in eye tastes whereas, 4 weeks PI.

Mild lyrrphocytic infiltration was detected in 1 / 18 (5.6 %) mice of group I and in 3 / 15 (20 %) mice of group E with no detection of the parasite in eye tastes.

On the other hand, the immurc-luorescence staining of eye tissues revealed few immunofluorescent deposits in the retime layers of 2 / 18 mice of group I (11.1 %) and marked immurc-luorescence deposits in 5 / 15 (33.3 %) mice of group E.