Organ and tissue culture of acacia senegal (L.) willd
Joint Authors
Source
Sudan Journal of Basic Sciences. |n Series : Biological Sciences
Issue
Vol. 1, Issue 1 (31 Jan. 2001), pp.69-88, 20 p.
Publisher
Sudan Institute for Natural Sciences Publication Unit
Publication Date
2001-01-31
Country of Publication
Sudan
No. of Pages
20
Main Subjects
Topics
Abstract EN
In vitro shoot multiplication and callus culture of Acacia Senegal (L.) Willd .
have been demonstrated .
Explants were taken from apical and axillary shoots (young and mature).
Mu-rashige and Skoog basal medium containing 1-naphthalene acetic acid (NAA), N6-benzyl aminopurine (BAP) and kinetin at 0.5-2.5 mg/1 each was used.
Shoot regeneration was obtained but the multiplication rate was low (xl and x2).
Also, the shoots regenerated showed a high mortality rate due to the small number of shoots produced per explant.
Explants of different ages and origins were used for callus initiation.
The callus was formed at the basal cut end of the explant with varying intensities and coloration with the various concentrations of NAA and kinetin used at 0.5 -2.5 mg/1.
Subsequent sub-cultures showed a high capability of A Senegal callus tissue for differentiation and development.
Ovulation, proembryos, endospermic cells and whole mounts of mature embryos were clearly demonstrated.
Plant tissue was maintained in culture for up to 12 months through subcultures every four weeks without apparent loss of viability.
Also, juvenile tissue was found generally more responsive in culture compared to mature tissue.
American Psychological Association (APA)
al-Tijani, S.& Ali, Y. H.. 2001. Organ and tissue culture of acacia senegal (L.) willd. Sudan Journal of Basic Sciences. |n Series : Biological Sciences،Vol. 1, no. 1, pp.69-88.
https://search.emarefa.net/detail/BIM-271150
Modern Language Association (MLA)
al-Tijani, S.& Ali, Y. H.. Organ and tissue culture of acacia senegal (L.) willd. Sudan Journal of Basic Sciences. |n Series : Biological Sciences Vol. 1, no. 1 (Jan. 2001), pp.69-88.
https://search.emarefa.net/detail/BIM-271150
American Medical Association (AMA)
al-Tijani, S.& Ali, Y. H.. Organ and tissue culture of acacia senegal (L.) willd. Sudan Journal of Basic Sciences. |n Series : Biological Sciences. 2001. Vol. 1, no. 1, pp.69-88.
https://search.emarefa.net/detail/BIM-271150
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 85-88
Record ID
BIM-271150