Molecular identification and in-vitro susceptibility of candida albicans and c. Dubliniensis isolated from immunocompromised patients

Abstract EN

Background : yeasts are increasingly implicated in serious systemic infections.

The aim of this study was to identify Candida alb cans and C.

dubliniensis from isolates of immunocompromised patients and evaluate the in vitro antifungal activities of them against antifungal agents.

Methods : One hundred and seventy eight C.

alb cans were isolated by routine methods from 403 immunocompromised patients.

All isolated C.

alb cans were inoculated on CHR OMagar Candida medium.

The carbohydrate assimilation patterns of all the isolates were studied, using the API 32C system.

To identify C.

alb cans and C.

dubliniensis, PCR was done by specific primers.

The susceptibility test for the isolates was performed by a broth micro dilution assay, according to the Clinical and Laboratory Standard Institute.

Results : Of 178 isolates C.

alb cans, six were C.

dubliniensis with PCR assay, and 7 % were resistant to amphotericin B, 4.6 % to fluconazole, 7 % to itraconazole, 1 % to nystatin, 2.3 % to voriconazole, and 7 % to ketoconazole.

None of the C.

dubliniensis isolates were resistant to the six anti-fungal agents.

Conclusion : It would be convenient to carry out antifungal susceptibility studies in order to establish the in-vitro activities of antifungal agents against local isolates and also to detect shifts toward resistance as early as possible.