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Assessment of piwil2 gene expression pattern upon germ cell development from mouse embryonic stem cell
Joint Authors
Makoolati, Z.
Movahedin, M.
Forouzandeh Moghadam, M.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 11, Issue 4 (31 Oct. 2009), pp.408-413, 6 p.
Publisher
Publication Date
2009-10-31
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Topics
Abstract EN
Background : In order to improve culture conditions for optimal spermatogenesis, quantitative assessment of the male germ cell gene expression profile upon spontaneous ES cell differentiation is necessary.
In this study, the quantitative expression profile of Piwil2, germ-line specific marker, during the early stage of embryoid body (EB) formation and differentiation (0-3-day-old EB) was studied. Methods : CCE mouse embryonic stem cells (ESCs) were cultured in DMED containing 20 % fetal bovine serum (FBS) for 1, 2 and 3 days.
The total RNA was isolated from ESCs, 1-3-day-old EBs, and adult testis as positive control.
cDNA was prepared and quantitative real-time PCR was done for Oct-4 to study the pluripotency of this cell line.
Also, the molecular pattern of Piwil2 expression in developing EB was investigated. Results : Our quantitative results confirmed the pluripotency of CCE mouse ESC line and showed that Piwil2 was expressed in undifferentiated CCE mouse ESC line.
Our results also showed that expression of Piwil2 increased significantly during the process of EB formation and differentiation up to 2-day-old EB and decreased non-significantly in 3-day-old EB.
Conclusion : Our results suggest that EB provide a cellular environment similar to the early embryonic microenvironment and cause the efficient and progressive germ cell lineage differentiation in this system.
American Psychological Association (APA)
Makoolati, Z.& Movahedin, M.& Forouzandeh Moghadam, M.. 2009. Assessment of piwil2 gene expression pattern upon germ cell development from mouse embryonic stem cell. Iranian Red Crescent Medical Journal،Vol. 11, no. 4, pp.408-413.
https://search.emarefa.net/detail/BIM-27221
Modern Language Association (MLA)
Makoolati, Z.…[et al.]. Assessment of piwil2 gene expression pattern upon germ cell development from mouse embryonic stem cell. Iranian Red Crescent Medical Journal Vol. 11, no. 4 (Oct. 2009), pp.408-413.
https://search.emarefa.net/detail/BIM-27221
American Medical Association (AMA)
Makoolati, Z.& Movahedin, M.& Forouzandeh Moghadam, M.. Assessment of piwil2 gene expression pattern upon germ cell development from mouse embryonic stem cell. Iranian Red Crescent Medical Journal. 2009. Vol. 11, no. 4, pp.408-413.
https://search.emarefa.net/detail/BIM-27221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 412-413
Record ID
BIM-27221