Anovel immunological technique for identification of human seminal fluid

Abstract EN

An immunological technique was investigated for the detection of human semen in forensic analysis.

This technique included a preparation of anti-human seminal plasma antibodies, by immunizing rabbits with treated human semen.

The human semen was treated with an acid to prevent cross reactivity with other human body fluids.

The antibody produced was tested against different animal's seminal fluid samples (dog, goat, sheep, and cow) and human body fluids (saliva, blood, vaginal fluid, ear wax and human semen).

It was found that using this developed technique was only selectively response with human semen.

The prepared kit was evaluated and tested in Forensic laboratory- Ministry of Health.

Finally, results were obtained in a comparison with the recommended techniques.